Journal: Cell Death & Disease
Article Title: Colorectal cancer patients-derived immunity-organoid platform unveils cancer-specific tissue markers associated with immunotherapy resistance
doi: 10.1038/s41419-024-07266-5
Figure Lengend Snippet: A Immunohistochemical staining of CD3, CD4, CD8, CD68 and Ki-67 in CRC tissues showed the presence of T-cells populations (CD4 + , CD8 + ) and macrophages (CD68 + ) in MSI and MSS patients. Ki-67 was used as a control proliferation marker of tumor cells; ( B ) Quantification of positive cells/area was performed with QuPath Software. Significance shown refers to Wilcoxon test p -values *< 0.05, **< 0.01, ***< 0.001.
Article Snippet: T-cells and Myeloid Derived Suppressor Cells (MDSCs) were isolated from patient peripheral blood mononuclear cells (PBMCs) by CD3 + immunomagnetic negative selection (#17951, StemCell Technologies) and CD33 + immunomagnetic positive selection (#17876, StemCell Technologies) following positive selection with CD3 + immunomagnetic positive selection (#17851, StemCell Technologies), respectively.
Techniques: Immunohistochemical staining, Staining, Control, Marker, Software